Validation of Spinal Cord Injury Sensory Deficit Model with Head-Fixed Rat

Background/Objective: Numerous studies have reported restoring motor function through spinal cord stimulation in a rodent injury model; however, replicating sensory information has not been thoroughly tested.We previously trained rats to detect artificial sensations which lead the question if this can be generated injured rat. Our study begins address by first validating model test. hypothesis is decrease percent correct response compared pre-procedure observed, then will verify loss dorsal column lesion of injury.
 Methods: We use detection head-fixed rat deficit model. begin placing water-deprived into custom-made head-fix set up where two water spouts are placed front Vibration stimuli applied hind paw via vibration at 200 hz ± 50 hz. The associate stimulus with left spout and no right spout. Correct recorded once demonstrates detection, undergoes hemi-section procedure. Post procedure, back repeat behavioral experiment.
 Results: Thus far, only habituation training completed following steps include training, hemi section, re doing measure difference rate.
 Conclusion Potential Impact:This crucial step for research as confirms allow further testing restore sensation. After validation, we sensation using cord.